FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9348492
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140360.15014186245225433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACG15900.020.262530
ATCCGAC22700.020.05100338
GTTAATC66100.019.8432987
GTATCCG23000.019.48647536
TCATTAC101250.019.138212
TATCCGA23700.018.61434637
TTAATCA104300.018.5784518
AGTTGAC52600.018.50481237
GTTGACA54150.017.97483638
GACATGT55150.017.70888741
TCCGACA25850.017.60689539
TAATCAT112700.017.2557959
TCCGACC30050.017.2266679
TGTCCTA57050.017.17522845
ACATGTC58550.016.8591742
ATCATTA116750.016.74707411
CAAACCG20150.016.49045410
CGCGGAC19250.016.37115156
CCACGCG20350.015.989675551
GATGTAT61650.015.95772469
Produced by FastQC (version 0.11.5)