FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8729666
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134820.15443889834960467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAATC56350.019.565617
ACCGACG15550.018.9065930
ATCCGAC21550.018.83963838
GTATCCG21900.018.53854836
TCATTAC86200.018.271212
AGTTGAC45350.017.90492637
TTAATCA91950.017.3194458
TATCCGA23550.017.23966837
TACCTCG13650.016.92283416
TCCGACC31450.016.8045949
GACATGT49800.016.37526741
TCCGACA25050.016.34707339
TAATCAT99000.016.298129
AGTGCGA11400.016.27197661
CTAGTCG6100.016.06561965
TGTCCTA51600.016.00752345
CCACGCG18700.015.90895451
GTTGACA50850.015.83064738
CTAGACG11500.015.82622366
ACATGTC52800.015.71000242
Produced by FastQC (version 0.11.5)