FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10986977
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTCG35950.025.0077114
TCGGCCC44300.024.3973758
TTCGGCG48950.021.93803820
AATCGCG14250.021.85293850
CGGGATT50350.021.60514515
CGCGGCA28700.021.59751732
ACCGACG22750.021.24165530
TCGTACG13050.021.17429213
TCGGCGA51750.020.75133321
ACCCGCG30750.020.59980225
CCCGTCG25850.020.57779344
TCGCCGG26000.020.4562848
GCGAGTG54100.019.91876424
CCGTCGC26750.019.88473145
GGATTCG55600.019.69109217
ATTCGGC55750.019.5131619
CAGTCCG33250.019.4636276
GGCGAGT56150.019.37609323
CAAACCG26250.019.32088710
CCGGGAT57600.019.24992814
Produced by FastQC (version 0.11.5)