FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10213451
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCCC40100.024.090178
ACCGACG22050.023.6503730
CAAACCG24350.021.70445410
TCGCCGG20700.021.64233648
TCCCTCG33200.021.6126464
AAACCGC25400.021.08281511
TTCGCGA18600.021.07509856
CGGGATT47500.020.77908315
TTCGGCG46550.020.75150920
AATCGCG12000.020.70806350
TCGTACG14250.020.386212
CCGTCGC22600.019.66788545
TCGCGAT20500.019.63398757
AACCGCC27800.019.51461212
CGACCGT11500.019.47791320
CGACGAG28350.019.3825632
CCCGTCG22900.019.25739344
GACGAGC28050.019.0906633
TCGGCGA51100.019.04075421
CGCGGAC27950.019.03383456
Produced by FastQC (version 0.11.5)