FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10370936
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222440.21448401571468576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGATC24500.024.85688643
ACGGCAC25700.023.1520710
AAACTCG26600.022.76270555
ACTCGTT27250.022.6053857
TCGGCCC38300.022.207118
TAGATCG23700.022.15220310
AGCGCCG39650.022.06818861
CTCGGGT40250.021.912548
ATCACGG27000.021.908137
ATCGTTA40900.021.90739370
CGGATAC34850.020.489676
TCCCTCG31050.020.2914074
TAGGGAC91000.020.15412370
GTCGTTA24900.020.09990335
AGTCTCG44250.019.9317145
TTCGGAT30600.019.90175442
GATTACC32050.019.76578727
TCGGCGT26400.019.4893748
CCATCGT45050.019.42303868
TCACGGC31050.019.3888178
Produced by FastQC (version 0.11.5)