FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9797296
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTCG28550.026.0978974
CGCGGCA25200.025.29024732
ACCCGCG25600.025.15580625
TCGGCCC38950.024.6955998
TCGCCGG25300.023.92932948
CCCGTCG26450.023.15696544
CCGTCGC26250.022.93234345
CGACCCG28500.022.8326123
CAGTCCG29300.022.2063586
AGTCCGC30750.021.4986427
CGGCACC30250.021.2995434
TTCGCGA20150.021.02580356
TCGCGAT20750.020.75828257
GCGGCAC31850.020.33948933
CCCCGAC35200.020.07431420
TTCGGCG47850.019.88469720
TCGGCGA48850.019.83649621
CGGGATT48100.019.63469115
GACCCGC33050.019.48050924
ATCGCGT22200.019.40135831
Produced by FastQC (version 0.11.5)