FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9915760
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206630.20838543893761044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGC62300.029.42212520
TACCTCG64700.029.08619316
GTGCGCA60100.028.75692623
ACCTCGG69700.026.84911317
CTCGGTG69700.026.74995619
GGTGCGC65100.026.65385222
CGGTGCG62850.026.4379621
CCTCGGT71200.026.33352718
TAGTACC74300.025.62839141
ATAGTAC76550.024.92397740
AAGGTCC75850.024.86665348
GTACCAA76100.024.83695843
TCATTAC147300.024.41115612
TTAATCA148500.024.2608818
CATTACC103450.023.90489813
GCGCAAA73650.023.80647725
TGCGCAA74200.023.76707524
GGTCCTA79900.023.56014850
CGCAAAC77800.023.39422626
GCAAACC79250.023.36521727
Produced by FastQC (version 0.11.5)