FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9947745
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA28200.028.55955932
TCGGCCC50200.025.6422448
CCGTCGC29100.025.49478345
TCGCCGG30100.024.87780648
CCCGTCG30150.024.6081444
ACCCGCG32600.024.58560625
CGACCCG36350.022.6190123
TCCCTCG46350.021.5855524
GCGGCAC38550.021.25504933
TTCGCGA23200.021.128156
CGGCACC37800.021.1211734
TTCGGCG60750.020.84587120
CCCCGAC40100.020.67577420
AGTCCGC40900.020.5257917
GACCCGC40300.020.31754524
CGGGATT63400.020.2484115
ACCGACG11350.020.05310230
TCGCGAT24350.019.99059757
CAGTCCG42500.019.9193676
TCGGCGA64400.019.7734321
Produced by FastQC (version 0.11.5)