FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7413542
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138920.18738681186401857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGTTA33850.025.6431770
AGCGCCG32700.025.47428961
CGGATAC26500.025.0954656
CTCGGGT33250.024.94697248
AGTCTCG34750.023.97100445
TAGATCG17000.023.67609410
CCATCGT36700.023.5561268
GCGATTT36500.023.20542127
TCTCGGG37150.022.7048947
GTCTCGG36700.022.6973446
TCGGGTG36800.022.6355149
GATACAC37750.022.437038
ACAGCGC37800.022.03698359
TCGACGT21000.021.999815
ACAGCGA38700.021.88625324
CAGCGAT38850.021.80174825
GACGTGT22100.021.6967817
GACAGCG39000.021.62815358
GCGGATA28500.021.3693795
AAGCGCG21750.021.24132737
Produced by FastQC (version 0.11.5)