FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10132248
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCCC29100.019.364458
TCCCTCG23650.017.6122974
GCGAGTG33850.016.23322124
TCGGCGA34400.016.17708621
TTCGGCG35800.015.34893420
ATTCGGC36100.015.31833219
CGGGATT37000.015.04039215
CGAGTGC37650.014.78072925
GGCGAGT36900.014.70182423
GGATTCG38450.014.29114517
CGTTGGA21150.014.231606533
CCGGGAT40300.014.06940814
CGGCGAG39500.013.91125422
GATTCGG41300.013.38969718
GTACCGC19700.013.32480635
AAACGGG21700.013.06445139
CCCGTCG17300.012.94778444
TGTACCG20250.012.79005934
TACCGCC19550.012.71087136
ACCCGCG18350.012.397696525
Produced by FastQC (version 0.11.5)