FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10960848
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136990.12498120583370922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACG17800.020.25299646
TCGTACG14400.018.47239312
AACGGCG20300.017.58628849
TCGTTTG38150.016.9721717
TCGATCG23550.016.64656
GTTATCG10850.016.4526656
ACCGACG15150.016.40234230
GTTCGCA20500.016.2192216
CTCGTTT41550.015.83605616
TCGGCCC34250.015.4310818
GTACGAG17050.015.39568714
GTTCTCG43650.015.15443313
CGACCGT23850.015.11496220
TCGGAAC24650.014.76680445
CAACGGA29900.014.7488321
AACGGAG29700.014.73050822
GTTCGAC25050.014.67039217
GCAACGG29600.014.5435920
GTCGTAC20500.014.51226311
ACCCGCG23450.014.47754925
Produced by FastQC (version 0.11.5)