FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10638732
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG138480.13016588819043473No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA135100.12698881784032157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTCG70950.027.5147614
ACCCGCG45400.026.74994925
TCGGCCC91100.026.0724778
CGTACGT5450.025.67501419
CGCGGCA47350.025.0691432
ACCGACG37900.024.94422330
AAACCGC38700.024.76683211
CAAACCG39000.024.30723810
CAGTCCG49900.024.1881436
TCGCCGG41500.024.1153648
AGTCCGC50600.023.919747
CCGTCGC42800.023.87511845
CCCGTCG44100.023.80603644
TTCGCGA38350.023.46272756
TCGCGAT38950.023.10531857
ATCGCGT41200.022.6068531
AACCGCC42500.022.470512
CCCCGAC54150.022.22343820
CGTCAAC42000.022.17593235
GCGTCAA42150.022.0974334
Produced by FastQC (version 0.11.5)