FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9328426
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111840.11989160872370108No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCCC40900.020.0125438
CGTACGT4100.019.6242619
TCCCTCG31800.018.2615324
ACCCGCG23900.017.42556825
CGGGATT48050.017.03470215
CGCGGCA24000.016.92493232
ACCGACG12350.016.72872730
TCGCCGG21050.016.62333948
TTCGGCG49550.016.52043520
CAGTCCG24450.016.0249046
TCGGCGA51650.015.91672921
GCGAGTG51450.015.91316624
CCCGTCG22050.015.87114944
CCGGGAT52450.015.672276514
CCGTCGC21450.015.66240845
CGGCACC26100.015.42874134
CGAGTGC53650.015.26455125
GGCGAGT53700.015.1796323
CGACCCG26550.015.02172723
GCGGCAC26850.014.86751633
Produced by FastQC (version 0.11.5)