FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8696135
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106160.1220772216622672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCCC41050.021.9977788
CCGTCGC20050.020.24922845
CGCGGCA22300.019.93235632
TCGCCGG21100.019.9049548
ACCCGCG23450.019.40272925
CCCGTCG21050.019.12099644
CGGGATT47900.018.99820915
TCCCTCG33350.018.8914994
TTCGGCG48400.018.58425120
TCGGCGA50300.018.09100321
GCGAGTG50850.017.75787724
CCGGGAT51200.017.70535314
GGCGAGT52350.017.182123
CGAGTGC52300.017.13170425
ATTCGGC53550.016.86242519
GGATTCG53550.016.79735817
CAGTCCG25650.016.7845616
CGGCGAG54650.016.5870622
GCGGCAC27150.016.50060833
AGTCCGC27500.016.1637947
Produced by FastQC (version 0.11.5)