FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6578994
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGC21350.023.59349811
CAAACCG21250.023.53991110
ACCGACG21000.023.5116630
ACCCGCG18700.021.89693325
CAGTCCG19350.021.6962226
AACCGCC23550.021.6861812
CGCGGAC22750.021.39197256
TCGCGAT11350.020.98052457
CCCGTCG16650.020.80815544
CGGCGAC23400.020.7950670
AGTCCGC20200.020.780427
TCGAACC23800.020.7247719
TTCGCGA11350.020.667956
ACGAGCT23950.020.61612134
GACGAGC23850.020.40906533
CCGTCGC17400.019.91125145
CGACGAG24450.019.76485432
CGCGGCA20400.019.74063332
TCCCTCG25100.019.652724
ACACCGA25550.019.32112328
Produced by FastQC (version 0.11.5)