FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9236673
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT409690.4435471516638079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACG19050.030.13086930
CGGCGAC20650.027.28854470
CGACGAG22000.025.93150332
CGCGGAC22650.025.34171956
AAACCGC23450.024.77608711
CAAACCG23750.024.61049310
AACCGCC23900.024.60274512
TCGAACC23600.024.47017319
CCACGCG23600.023.72865351
ACACCGC24350.023.5729961
ACCGCGG25300.022.96452163
GACACCG25400.022.73631560
GACGAGC25500.022.0977733
ACCGCCT26000.022.07713313
TTCGGCG22850.022.05662520
ACCCACG25800.021.97671549
CACCGCG26250.021.86675662
CGGGATT23900.021.52728715
GCGCGGA26800.021.41753655
TCGGCGA24050.021.24714321
Produced by FastQC (version 0.11.5)