FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11365201
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGCG29500.023.96367625
CGCGGCA28300.023.880432
TCCCTCG37650.022.7662474
TCGCCGG24950.022.7212548
CCCGTCG25900.022.56548344
CCGTCGC26650.022.06087945
TCGGCCC50450.021.7730228
CGACCCG32700.021.29163623
TTCGCGA24150.021.16743556
CAGTCCG32800.021.0127416
AGTCCGC34200.020.7644377
ACCGACG17450.020.46719230
TCGCGAT24850.020.4326157
ATCGCGT25600.020.38042431
CCCCGAC34600.019.91880420
GCGGCAC35000.019.80889333
CCCCCGA35950.019.36535319
CGTCAAC27850.018.9839335
GACCCGC36150.018.97122824
CGGGATT58350.018.76521915
Produced by FastQC (version 0.11.5)