FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10590388
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGCA28150.025.61198632
ACCCGCG29150.025.21421225
TTCGCGA24600.024.61339856
TCGCCGG26450.024.34752548
TCGCGAT25150.024.21406757
CCGTCGC26950.023.63617945
CCCGTCG27700.023.24891744
ATCGCGT26350.023.11155731
CAGTCCG32200.022.9367716
TCGGCCC43800.022.853928
GCGGCAC31900.022.820633
TCCCTCG34150.022.7544254
CGACCCG33100.022.20537223
AGTCCGC33650.022.1544677
CCCCGAC34050.021.79131520
GCGTCAA28450.021.6513434
CGTCAAC28850.021.4728735
CGGCACC34050.021.1740834
CCCCCGA36350.020.31621219
CATCGCG30500.020.19637530
Produced by FastQC (version 0.11.5)