FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11055603
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAACG13800.023.8351946
ACCGACG18950.023.46825630
AATCGCG10900.023.1113250
TCCCTCG30000.022.506184
TCGTTTG42700.022.4462217
AACGGCG15000.021.69342649
CGCGGCA23950.021.20082332
TCGGCCC38250.021.0338
TTCGCGA26200.020.98249456
TCGCGAT26650.020.89447457
TCGTACG10050.020.88295712
GTTCTCG46850.020.83058213
CGACCGT16150.020.57734320
AAACCGC21550.020.45163711
CAAACCG23000.020.3789510
CCCGTCG22500.020.21966444
CGACGAG22350.020.2116832
CTCGTTT48650.020.06050716
GCGTCAA29950.019.99353234
CGTCAAC29950.019.75799235
Produced by FastQC (version 0.11.5)