FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10282880
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104120.10125567934275222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGAT21900.024.77153257
TTCGCGA22200.024.27924356
TCGGCCC37100.023.7740148
TCGCCGG20500.023.04885948
CGCGGCA23150.022.52652532
CGCGATG24600.022.19476558
CGTCAAC26450.021.833335
TCCCTCG32400.021.7139874
CGGAACG11750.021.4468846
CCGTCGC22050.021.27001645
AACGGCG11900.021.17654249
GCGTCAA26850.020.98662834
ATCGCGT25850.020.98612431
CGGGATT42650.020.92611315
ACCGACG16100.020.65184230
CCCGTCG23250.020.62372644
TCGGCGA43200.020.33532321
TCGTTTG38300.020.19559917
GCGAGTG43900.020.01126324
AATCGCG11200.020.00016650
Produced by FastQC (version 0.11.5)