FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10794740
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147260.13641829261288368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGCA27350.016.38004116
ACCCGCG19300.015.59579625
TCCCTCG24950.015.2915144
TCGGCCC29600.015.2538868
CCCGTCG19050.014.88190144
CCGTCGC18850.014.66844645
CGACCCG21000.014.33327923
CAGTCCG22000.014.3193216
TGTTCGC33050.014.19036915
CGCGGCA21300.014.13107432
GTGTTCG32350.014.06473114
ATCCGAC9500.013.99988138
TCGGCGA31850.013.95579621
ATTGCCG19650.013.89307662
ATCGCGT13150.013.83999531
GTCCCGT12400.013.8318741
TGCCGAC20350.013.75916164
TCGCCGG20700.013.69566348
CGGGATT32450.013.48204115
AGTCCGC23400.013.3123467
Produced by FastQC (version 0.11.5)