FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633594
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTCTCACTCAAACCGCCTCGAACCCCACACCGACGAGCTCCCTCAGGAC30490.1866436825796373No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG19560.11973599315374565No Hit
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC17350.10620753993954433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGA350.001256161639.99846367
AATCGCG1650.038.18268650
AAACCGC11100.035.00186511
CGGCGAC11300.034.6889370
CAAACCG11150.034.52993410
CGACGAG11100.034.3680532
ACCGACG11000.034.36231230
CGCGGAC11500.033.4769756
ACACCGA11400.033.1566228
GCCGTTA851.0655458E-732.94091843
TTACGAG4800.032.81324822
GACGAGC11750.032.76469833
TCGAACC11800.032.6278619
GACACCG12150.031.9740860
CGAAACG552.9946808E-431.81695629
ATCGCGC2000.031.49878951
ACCGCGG12350.031.17289263
ACTAGCG450.004309831.10991366
AACCGCC12600.030.83592412
ACCCACG12300.030.73240749
Produced by FastQC (version 0.11.5)