FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4071389
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC61380.1507593600120254No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG56030.13761888141860185No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA46950.1153169102731279No Hit
GGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGG46900.11519410206197443No Hit
CTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG41090.10092378792593878No Hit
CGTCTCACTCAAACCGCCTCGAACCCCACACCGACGAGCTCCCTCAGGAC41050.10082554135701599No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGG14400.032.0775748
ATTGCCG6600.030.77435962
ACCCGCG17100.030.70076825
CCGTCGC15250.030.52398545
CCCGTCG15550.030.16054344
AGTCCGC19050.028.4641867
ACCGACG17050.028.33728430
CAGTCCG18800.028.2868846
AAACCGC17650.028.14528511
TCCCTCG23500.027.9891284
TTCGCGA10900.027.94312156
CAAACCG17700.027.67048810
TCGCGAT11450.026.60480157
ACGTACG2900.026.56262257
AATCGCG3050.026.38773350
ATCGCGT11700.026.33332431
GCGGCAC19750.025.88212833
AACCGCC19600.025.52423112
ACCCACG19350.025.13729749
GCGTCAA12400.025.12942334
Produced by FastQC (version 0.11.5)