FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3818737
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGCCGGGGGGGCTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGC59010.15452753096115288No Hit
GGGGACAGTCCGCCCCGCCCCCCGACCCGCGCGCGGCACCCCCCCCGTCG55640.14570262366850611No Hit
GGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGG45380.11883510176270322No Hit
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA43900.11495947482112541No Hit
CTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAG38450.1006877404754504No Hit
CGTCTCACTCAAACCGCCTCGAACCCCACACCGACGAGCTCCCTCAGGAC38430.10063536713840204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGG1055.529728E-1033.3326343
ATTGCCG6450.032.01566762
CCGTCGC14300.031.5727645
TCGCCGG14500.031.13849448
CCCGTCG14450.031.00280444
ACCCGCG16250.029.72244825
ACCGACG15700.028.9802830
AAACCGC16250.028.00050711
CAAACCG16600.027.40977710
AAGTTCG6050.027.1902215
CGGCGAC16900.026.7168170
GAGCGTA1056.7848487E-726.66610343
CGACCGT5800.026.5515120
GCGGCAC18200.026.53755233
CGACGAG17400.026.34967432
TTCGCGA9850.026.29420756
GACGAGC17200.026.04562233
TCCCTCG22750.026.0021744
AACCGCC17950.025.93293812
GCGTCAA10000.025.89911334
Produced by FastQC (version 0.11.5)