FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11604159
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCCC54750.023.8305978
TCCCTCG42850.023.022354
ACCCGCG28250.019.57304825
CCCGTCG26900.018.9948344
CCGTCGC27350.018.42598245
CGCGGCA29950.018.35644732
ACCGACG18250.018.03604730
CGGGATT72050.018.01158715
CCGGGAT74850.017.33773214
CCCTCGG57550.017.14195
TCGCCGG29350.017.0496748
AGTCCGC32650.017.0342927
TCGGCGA77050.016.98078721
CAGTCCG31700.016.8843926
GCGAGTG76750.016.8678224
ATTCGGC77700.016.74811219
CGACCCG33650.016.53149423
TTCGGCG79000.016.51693320
CGAGTGC79100.016.4583525
GGATTCG81150.016.29395717
Produced by FastQC (version 0.11.5)