FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12308811
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGCG21700.019.35329825
TCGGCCC39700.018.2388748
TCCCTCG30900.018.2269444
TCGCCGG24700.016.57560748
CGACCCG25550.016.56877123
CAGTCCG25250.016.3482256
ATTGCCG22300.016.17712662
CCGTCGC23800.016.02774645
CGCGGCA26550.015.95897832
TGCCGAC23950.015.49983164
TCGGCGA46100.015.40472321
CGACCTC23550.015.31466867
CCCGTCG25250.015.24649744
TTCGGCG46400.015.07826620
CGGGATT46200.015.06690315
GCGAGTG48350.014.763206524
AGTCCGC29000.014.715677
CCGGGAT48150.014.67465914
TACCTCG19600.014.63437716
ATTCGGC48450.014.51236319
Produced by FastQC (version 0.11.5)