FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11398391
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT412990.36232306822954224No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCGC44150.028.6799211
ACCGACG43600.028.51114530
CAAACCG44900.028.12295310
TCCCTCG44250.027.0362974
AACCGCC47250.026.72424712
CGACGAG48700.025.81332832
TCGAACC49650.025.57558319
TCGGCCC58250.025.3408058
ACCGCCT51250.024.7750613
CGGCGAC50650.024.74343970
GACGAGC51200.024.6894833
CGCGGAC50800.024.60697756
ACACCGA52550.024.05246528
TACCCGC15650.023.69144810
CGAGCTC54100.023.29995735
CCACGCG53700.023.26237751
ACACCGC54450.023.22010261
AATCGCG16050.023.10891350
ACGAGCT54750.023.08861234
GACACCG54450.022.96261460
Produced by FastQC (version 0.11.5)