FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9222307
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTA95810.10388940641425189No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTCG35100.031.3114664
AATCGCG11100.030.5851650
TACCCGC11700.029.91492310
TCGGCCC44000.029.034168
CGGAACG13900.028.45345746
ACCGACG24800.027.52007730
AACGGCG14750.027.28790349
GTTCTCG43500.025.10377913
CAAACCG28300.024.85898610
TCGTACG10600.024.76447712
AAACCGC28500.024.6846711
CGACCGT16650.024.38417620
TCGTTTG43700.024.3477517
ATCGCGC13800.024.34735551
TTCGCGA22300.024.32714856
TCGCGAT22700.024.20684257
CTCGTTT45200.023.4625716
TCGGAAC17050.023.40194145
GTTCGAC18100.023.39771717
CCGTCGC19300.023.21261445
Produced by FastQC (version 0.11.5)