FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005283000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005283000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10691266
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCCC42550.022.9354828
TCCCTCG32300.022.3103584
ACCGACG22500.020.3878630
CCCGTCG22950.018.90861144
CGGGATT51400.018.71409215
CCGGGAT52200.018.42737214
ACCCGCG26900.018.21430825
CCGTCGC24300.018.14565845
AAACCGC25450.018.14217811
TCGGCGA54600.017.87538721
CAAACCG25750.017.52329410
TCGTACG9650.017.39871612
CAGTCCG27800.017.36536
TTCGGCG55950.017.25594120
TCGCCGG24250.017.17105348
AGTCCGC29200.017.1298837
CGACCCG29050.017.10096523
AACCGCC27400.016.85103612
GCGAGTG57500.016.7337824
ATTCGGC58500.016.6830719
Produced by FastQC (version 0.11.5)