FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005283001

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005283001
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9932956
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTCG31600.020.9345844
TCGGCCC41150.020.4133158
TCGCCGG22100.018.6877748
CCCGTCG22650.018.54303444
CAGTCCG25200.018.3344296
CCGTCGC24000.017.6459145
ACCCGCG25350.017.5342425
CGCGGCA26150.017.53300932
AGTCCGC27450.016.4480767
ACCGACG21600.016.36548230
CGGGATT50700.016.22327815
TCGGCGA51600.016.0754121
GCGGCAC29100.015.9961533
GCGAGTG52450.015.94827124
TTCGGCG52500.015.93316220
CCGGGAT52000.015.81785614
GGCGAGT54300.015.27600223
ATTCGGC53950.015.24551219
CGAGTGC55700.014.89204525
CGACCCG30450.014.59747123
Produced by FastQC (version 0.11.5)