FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005283004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005283004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10648527
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129170.1213031623998324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGCG19450.020.87357525
GCGTACG6000.020.41909442
CGTACGG6000.019.83457443
CGACCCG23250.018.20798123
TCGGCCC32250.018.1136748
GTTCGCA34400.017.99885416
CCGTCGC19950.017.5423245
CCCGTCG20600.016.81930744
CGCGGCA24950.016.42002532
AGTCCGC26400.015.9000027
CAGTCCG26650.015.6215696
GTGTTCG39650.015.61528514
CGCAGAC41200.015.3681519
TCGCAGA41000.015.35779418
TGTTCGC41150.015.30109415
TCGCCGG23150.015.26763348
CGTTAAC22250.015.26454567
GACCCGC27800.015.23011424
GCGTTAA21750.014.97215266
ACCGACG14300.014.93619730
Produced by FastQC (version 0.11.5)