FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005283006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005283006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12258974
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT493260.40236646231568807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCCC49050.023.0346168
CGGGATT51550.021.98519315
TCCCTCG39850.021.5075384
GCGAGTG53600.021.34787424
ACCGACG28650.021.26631730
TCGGCGA55600.020.63767821
GGCGAGT55200.020.59920123
CGAGTGC55950.020.39267525
GGATTCG56200.020.35327117
CCGGGAT57450.020.0927614
CGGATAC22450.019.9457386
TTCGGCG58450.019.51089320
ATTCGGC58300.019.38092419
AAACCGC33050.019.15687411
CGGCGAG62250.018.7708922
ACCCGCG31550.018.63659725
CAAACCG33600.018.42686710
TCGTACG11000.018.12590413
CGCGGAC33800.017.92053656
GATTCGG63700.017.84771718
Produced by FastQC (version 0.11.5)