FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005283008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005283008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11431304
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT474310.4149220421397244No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGACG30550.024.86041530
CAAACCG33400.023.36823310
TCGGCCC50550.022.156648
AAACCGC35000.022.10006711
TCCCTCG39450.021.560514
CGGGATT52050.021.38325715
CGACGAG36750.021.04731632
TCGGCGA54300.020.69030821
CGCGGAC36900.020.67756856
AACCGCC37850.020.52846512
CGGCGAC37450.020.46744270
GCGAGTG54950.020.25439624
TCGAACC38550.020.06473519
ACCCGCG28650.019.79020325
CCGGGAT57000.019.64918314
ATTCGGC56750.019.61222319
GGCGAGT57350.019.5288423
GGATTCG57150.019.413817
TTCGGCG57500.019.29554220
GACGAGC39750.019.2827433
Produced by FastQC (version 0.11.5)