FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005283011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005283011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8747451
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104130.11904039245261276No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCTA18850.019.3004213
ACCGACG20900.016.25016230
TCGTACG11000.016.21893313
TCGGCCC23850.016.1341698
CAAACCG21950.015.77767410
TCCCTCG20200.015.7603954
ACGTCCT24650.015.1848912
CAGTCCG17200.015.0515186
CGTACGA11850.014.76025914
AAACCGC23000.014.60110411
CGCGGAC24050.014.558516556
ACCCGCG17800.014.15658125
TCGAACC24250.013.99344419
GCGAGTG27800.013.84528524
AGTCCGC18600.013.7294227
CGACGAG25000.013.72542432
CCGGGAT28400.013.67246914
AACCGCC25650.013.63821112
GACGAGC25750.013.46162933
ACACCGA26600.013.29343328
Produced by FastQC (version 0.11.5)