FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005283013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005283013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8147687
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97520.11969041029681184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCTA17800.019.46612513
TCGTACG9900.017.67672312
TCGGCCC23650.016.7231048
ACCGACG18650.016.70237430
CGTACGA10750.016.60450713
GTCGTAC11800.015.72039711
ACGTCCT22800.015.65785612
CAAACCG20600.015.63122210
AAACCGC20550.015.32852411
CGCGGAC21300.015.11714856
TCGAACC20850.014.9399219
CCGGGAT27350.014.7165114
CGACGAG21850.014.57653332
AACCGCC21450.014.52210912
TTCGGCG27400.014.30644720
ACACCGA22550.014.12422128
TCGGCGA29150.014.04790721
TCCCTCG20050.013.9656524
GCGAGTG27650.013.92410224
GTCCTAC23300.013.6694114
Produced by FastQC (version 0.11.5)