FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005283017

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005283017
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9667490
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118610.12268955023485931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACG11250.017.42205412
AATCGCG9050.017.40305750
ACCGACG18300.017.21321730
CGTACGA11400.017.19281814
GCGTACG5300.016.50936142
TCGGCCC26400.016.4394918
TTCGGCG26750.015.96246320
TACCTCG14450.015.743875516
TCCCTCG19800.015.73298554
TGCCGAC15150.015.70958364
AGTCCGC20950.015.5370847
CGTACGG5200.015.480700543
ACCCGCG20150.015.45906925
GTCGTAC13600.015.44126711
CGCGGAC21450.015.17460156
CCGCTAA18700.015.1610673
CGGGATT29550.015.04215715
CGCGGCA20800.014.97589532
ATTGCCG15200.014.96703962
CAAACCG21600.014.90741810
Produced by FastQC (version 0.11.5)