FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005283018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005283018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12130351
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381260.3143025292508024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAC30150.021.5817266
ATCGTTA47100.021.18394570
TCGGCCC64750.019.7184358
GCGGATA30350.019.479985
CTCGGGT44200.019.47506948
CCATCGT51450.018.85015568
CGGGATT67450.018.7214415
CGACCCG42800.018.7187723
GCGAGTG67700.018.6571524
TAGATCG21250.018.60103410
GCGATTT48100.018.55686427
AGCGCCG49650.018.12946361
ACAGCGA49650.018.04045524
ACCCGCG45250.018.02084425
AGTCTCG49350.018.0118945
CCCCGAC47750.017.8756120
ACAGCGC50200.017.79033959
CCCGTCG40950.017.77602644
GATACAC50400.017.6981968
CAGCGAT51200.017.56713725
Produced by FastQC (version 0.11.5)