FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005283021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005283021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6172265
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87430.14164978334533596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTCG23950.031.85781116
GTGCGCA24600.029.7353223
CGGTGCG24550.028.51280221
CGCAAAC29350.027.54642926
TCGTTTG25700.026.9645717
TGCGCAA29300.026.39891424
GCGCAAA29500.026.33858325
TCGGTGC28200.026.31165320
GGTGCGC28150.025.86106122
TAGTACC31300.025.38299841
TACGCGA4300.025.23237816
ACCTCGG30800.025.11325517
CTCGGTG30600.024.247819
TCATTAC83500.024.22776412
CATTACC59150.024.14203513
TCGGCCC16600.024.0363648
CTCGTTT30250.023.0246316
TCCCTCG16700.022.8458144
GGTCGTT23000.022.82592245
TTAATCA88250.022.8047398
Produced by FastQC (version 0.11.5)