FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005283022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005283022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8727146
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99810.11436728570829455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACG11200.018.11439713
CGTACGA11800.016.89688914
GTCGTAC15500.013.314939512
GAGTCGT15950.013.15851710
GTCGATT17650.012.68495722
CCGCTAA19050.012.6704033
TCGGCCC21000.012.6593298
AGTCGTA16600.012.43255311
GTACGAG16500.012.29583315
TACGAGG17500.011.99318716
TACCTCG13150.011.70437716
AGAGTCG18000.011.6599099
TCCCTCG18650.011.6291834
TACGCGT6501.8189894E-1211.30256723
GCGAGTG22650.011.27763124
ACCGGGA17250.011.15960753
TAGTGCG18400.011.0398861
GGGCCTA15200.010.8170292
GCGTTAA14000.010.75529666
ACCGACG17600.010.74398930
Produced by FastQC (version 0.11.5)