FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005283023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005283023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8143579
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95670.11747905926865816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACG10050.014.2786513
CGTTGGA15100.012.74830433
CAAACCG19300.012.69441610
AAACCGC19050.012.30974811
TCGGCCC21750.012.2296968
CGTACGA12400.011.85487514
GCGAGTG22500.011.82189524
CGTTAAC12450.011.80719167
ACCGACG18100.011.79547130
CAGTCCG16350.011.5603076
GCGTTAA13350.011.27351166
GTCGTAC13350.011.27351112
TACCTCG13500.011.14811316
CGAGTGC23850.010.85930325
TCGAACC20650.010.84722319
CGACGAG21500.010.74415232
AGAGTCG16650.010.7205568
CCGGGAT24250.010.68044514
CCACGCG20800.010.600862551
CGCGGAC20150.010.59543556
Produced by FastQC (version 0.11.5)