FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005283024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005283024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7486597
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG21200.029.37149210
GAACACG24800.025.39254221
CTAGTCG17200.023.82119865
CGTCCCA26250.023.60016426
ACGTCCC26400.023.59675625
AGATTCG24550.023.23798653
TACCTCG22050.023.16315716
AACACGT27050.023.15215522
ACCCGAG27000.022.93249912
GATTCGA25500.022.65039454
GGTCGTT49800.022.4171445
CGAGGGG29350.022.40740815
GTCGTTT51550.021.92665546
TGTACCC29100.021.8786379
CGGTATA19200.021.6986670
GGGTCGT52550.021.57743644
GTGTACC30350.020.9775418
GTCGGTA19550.020.9541468
GCGAGCC20100.020.38431265
CCGAGGG33750.020.21169514
Produced by FastQC (version 0.11.5)