FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005283025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005283025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6996629
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG18250.030.87740310
CGTCCCA22300.024.32720426
GAACACG23400.023.93147521
ACGTCCC23550.023.77921525
GGTCGTT49600.023.56840145
TACCTCG20650.023.38985316
GATTCGA23200.023.23228354
AGATTCG23700.023.18550953
AACACGT24200.023.14051622
GGGTCGT50900.023.10398144
GTCGTTT51200.022.83205446
CGAGGGG25300.022.54959115
CAAGCGG54850.021.50350839
TGTACCC26550.021.4883929
GACCCGT5450.021.1948011
ACCCGAG26550.021.0924512
CTAGTCG15450.021.06737965
TCATTAC120450.021.06700112
CATTACC89300.020.4199513
ACAAGCG57850.020.3885238
Produced by FastQC (version 0.11.5)