FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005283027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005283027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9345759
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGATC17500.020.00000643
AAACTCG22150.016.74917255
ACTCGTT21300.016.26743757
ACGGCAC22000.015.90909610
CGGCACT22450.015.4342211
GATTACC26050.015.31670327
CGGATCA23850.015.11522844
AACTCGT22550.015.05519656
TTCGGAT23750.014.73676742
GACCCGT8750.014.4016221
ATCACGG25650.014.0545117
TCGGCCC20800.013.629818
ACCCGCG16200.013.61104225
TCACGGC25900.013.3783828
CGTTGGA17850.012.94104233
CGTTGAA27050.012.80961660
GAAACGG20700.012.68109638
AAACGGG18550.012.64137839
CTCGTTG29100.012.62866758
CCCCCGA27500.012.4725319
Produced by FastQC (version 0.11.5)