FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005283029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005283029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11610577
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACC38000.020.5442341
GTCCTAT54400.019.30103351
ATAGTAC41850.018.99082840
TTAATCA72850.018.782898
AATAGTA52500.018.0060339
ATTAATC68700.017.98187
GGTCCTA45350.017.82760250
CATGCTA60400.017.38333968
TCATTAC80500.017.04140512
GTCCCTA29200.016.9017581
TACCTCG47450.016.74233616
ATTACCT63200.016.55688514
TAATCAT87350.016.3059719
GTACCAA49050.016.27168843
TTACCTC60350.016.23705115
ATGCTAA65400.015.94730969
CCTATTA48450.015.6740474
CATTACC68150.015.61071913
AAATAGT65050.015.60874438
ATCATTA90300.015.57949911
Produced by FastQC (version 0.11.5)