FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005283030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005283030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9592933
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAG15600.021.3132821
GTACGTA17050.019.91135820
GTCCCTA19850.018.6951051
GCCCTAG29200.018.21894862
CCTAGGC29600.017.85450464
TAGGTAC23800.017.35281411
CCCTAGG30900.016.99016863
CGTACGT20950.016.70588519
TCATTAC59000.016.66936912
CATTACC47500.016.43145813
ATCATTA61400.016.41681711
CGATCTA20150.016.32691247
GTGTACC44400.016.08171833
ATTAATC54650.016.0106957
TTAATCA62100.016.0061578
CGCCCTA33100.015.96656661
CGACTAG22000.015.90897313
CCTATTA36800.015.884694
TAATCAT64500.015.8449279
CTAGGCT34350.015.79327165
Produced by FastQC (version 0.11.5)