FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005283031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005283031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10335560
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA16450.020.21115320
TACGTAG16750.019.84887321
CAAACCG48850.019.55812610
GTCCCTA23200.019.3118881
AAACCGC49400.018.8444711
CGTACGT20300.017.24000419
GACCCAC52650.016.9512848
GCCCTAG32350.016.6606762
ATTAATC56000.016.560867
TAGGTAC24200.016.4861311
CATTACC50750.016.34344513
ACCGACG56700.016.23492230
CCCTAGG34200.016.16869263
TCATTAC62600.016.15670212
TCATACG20650.015.93216629
ACACCGA58950.015.73348328
CCTAGGC35000.015.4991464
TTAATCA64300.015.4572558
ATCATTA67400.015.4214711
ACCCCAC60650.015.40662623
Produced by FastQC (version 0.11.5)