FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005283032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005283032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12178429
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCTA25600.018.735471
ATAGTAC34100.017.44774840
TAGTACC34900.016.84723941
GTCCTAT49300.016.68281751
TTAATCA71900.016.356058
ATTAATC61750.016.2105277
ACAGTGT60600.015.88251159
CATGCTA54750.015.66306668
TGAGTCT66150.015.6605443
AGACAGT65400.015.30537157
CTGAGTC67900.015.25691842
GGTCCTA43400.015.07996550
CCTATTA45950.014.7788734
TAATCAT81700.014.7368429
GAGTCTC68500.014.71454244
AGTCTCT59700.014.24547245
GAGACAG80850.014.24183956
CTATTAA56350.014.1013485
GGAGACA75800.014.03630555
TGGGGTG69100.013.8821411
Produced by FastQC (version 0.11.5)