FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005284301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48353078
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGA8810501.822117715029434No Hit
GTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGT6541851.3529335195579484No Hit
CAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAG5048931.044179648708196No Hit
CAAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTT3967950.8206199406788539No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA2921530.6042076576800343No Hit
GGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGT2638180.5456074585365589No Hit
CACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCA2492600.5154997578437509No Hit
TGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG2297850.4752231078236632No Hit
CACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGC2281570.4718562073752575No Hit
CCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACT2052980.4245810370127834No Hit
GCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGG2033770.4206081772084913No Hit
GGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACC1991980.4119655009346044No Hit
CCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTG1908010.39459949168075714No Hit
CCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTC1833260.37914028968331653No Hit
GTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCA1749170.3617494629814466No Hit
GTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAG1723620.3564654146732913No Hit
CATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATG1682810.3480254142249227No Hit
GAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAAGAG1407010.29098664618620557No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACAC1402790.29011389926407577No Hit
AATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATG1342960.2777403333041177No Hit
ACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTT1314630.2718813474501044No Hit
CCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCAT1311920.2713208867489263No Hit
GTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTT1267890.2622149514452834No Hit
TGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAAT1248800.25826690908901395No Hit
GGTTTGTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCT1217990.2518950293091993No Hit
GAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATT1144270.23664884374061979No Hit
GGGTTTGTTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTC1129560.23360663823717695No Hit
AGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTA1124300.2325188067655176No Hit
GATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCC1120140.23165846856739916No Hit
ATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGT1091720.22578086962736893No Hit
CCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTC1054920.21817018556709047No Hit
TGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAA1005040.2078543996723435No Hit
CTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGC972440.2011123262928577No Hit
TGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTG957230.1979667147559872No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG954550.19741245841681473No Hit
ATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGC931710.19268887080983757No Hit
GCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATT925300.1913632054612945No Hit
TATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAA886890.1834195539733789No Hit
CAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA881070.18221590774428054No Hit
GGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACC854760.17677468226531515No Hit
GGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA795400.16449831797677905No Hit
CAACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACC786800.16271973420182267No Hit
AGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTG786570.16267216742644594No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGACACATGTT765150.15824225295440345No Hit
AGGAGAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGA764280.15806232645623924No Hit
CATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCT755730.15629408328462566No Hit
AAAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCCTTCCATGGTTTCCTCCCTTCCTTA753130.15575637191080163No Hit
AAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGT752660.15565917023937959No Hit
CGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTGAATTGCAGGACACA727710.15049920917133755No Hit
CTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGACCCTATGG712860.14742804997853498No Hit
CAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCA711970.14724398723903367No Hit
CAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAA701520.14508280114039482No Hit
CAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTCCCTC669680.13849790493171912No Hit
AACACAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCA669170.1383924307776229No Hit
CTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGTTAAAA661680.13684340839687598No Hit
GTTTACCAAAAACATCACCTCTAGCATCACCAGTATTAGAGGCACCGCCT637670.13187785067167804No Hit
GTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA622710.12878394215152136No Hit
GGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCG614130.1270094946179021No Hit
GTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCAAGACGAGAAGACCCT612190.1266082792082026No Hit
AGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAA592390.12251340028446586No Hit
GGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAG578030.11954357900442245No Hit
CGGTACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGG576390.1192044072147796No Hit
CTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGG576210.11916718104274561No Hit
ACTGAAGATCCTGGTGTCGCCATGGGCCGCCGCCCCGCCCGTTGTTACCGGTATTGTAAGAACAAGCCGTACCCA573410.11858810725555052No Hit
CACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATGACACAGCA559310.11567205711288948No Hit
CCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAG550650.11388106461392179No Hit
CATAATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACC544630.11263605597145233No Hit
CTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACCTGT543140.11232790599183778No Hit
AAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTACCAAAA533940.11042523497676818No Hit
GGCAAATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCACC533750.11038594068406567No Hit
GGGAAGATGGCGGACATTCAGACTGAGCGTGCCTACCAAAAGCAGCCGAC518290.10718862613048129No Hit
AAGAGATAAATCAAATCTACTTTCGGATTAGCCCAGATGTAAGAGATGCC510920.10566442119775706No Hit
ATCACTTGTTCCTTAATTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG502120.1038444750094296No Hit
GGAAAAGAAACTAACCAGGATTCCCTCAGTAACGGCGAGTGAACAGGGAA501970.10381345319940129No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG499330.10326746934290305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)