FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005284306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58821657
Sequences flagged as poor quality0
Sequence length35-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAGGT908760.15449411770226057No Hit
CTCGTCTTGCTGTGTCATGTCCGCCTCTTCACGGGCAGGTCAATTTCACT894760.15211404194206904No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGT782720.1330666356440792No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGATCATCAATGGCTGACGGCAGTTGC740700.12592300825527578No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT740350.12586350636127097TruSeq Adapter, Index 6 (97% over 36bp)
GTCCCAGATTTATTGAAAATAATACAGCACTACAGAAAAAATTCAAACAG704720.11980621355158357No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCG622560.10583856894748817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)