FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005284312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005284312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9385107
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA493954552.63173877506138No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTCGGGTGCCAA3389763.6118501366047293No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTTGGAATTCTCGGGTGCC1261761.3444279324678983No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACATGGAATTCTCGGGTGCCA616240.6566147834009778No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG563930.6008775392757909No Hit
TCTTAACCCGGACCAGAAACTATGGAATTCTCGGGTGCCAAGGAACTCCA543440.5790450764173494RNA PCR Primer, Index 1 (100% over 28bp)
TCCTGTACTGAGCTGCCCCGAGTGGAATTCTCGGGTGCCAAGGAACTCCA488200.5201858646896621RNA PCR Primer, Index 1 (100% over 28bp)
AGGTTCCGGATAAGTAAGAGCCTGGAATTCTCGGGTGCCAAGGAACTCCA470190.5009958863548386RNA PCR Primer, Index 1 (100% over 28bp)
GTCCCACTCCGTAGATCTGTTCTGGAATTCTCGGGTGCCAAGGAACTCCA422760.45045836984064214RNA PCR Primer, Index 1 (100% over 28bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTTGGAATTCTCGGGT310820.33118429017378276No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTATCGGGTGCCA302400.32221262900891806No Hit
TGAGGTAGTAGATTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA296070.31546790036597344RNA PCR Primer, Index 1 (100% over 28bp)
TGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA254270.27092924992757145No Hit
TTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCC250630.26705076457839No Hit
CCCCCCACTGCTAAATTTGACTGGCTTTGGAATTCTCGGGTGCCAAGGAA223240.2378662278437529RNA PCR Primer, Index 1 (100% over 23bp)
ATTGGTCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGC219570.2339557769559793No Hit
TCCTGTACTGAGCTGCCCCGAGATGGAATTCTCGGGTGCCAAGGAACTCC209020.22271456255107158RNA PCR Primer, Index 1 (100% over 27bp)
TGAGGTAGTAGGTTGTATAGTTTGGAATTCTCGGGTGCCAAGGAACTCCA201180.21436090179898856RNA PCR Primer, Index 1 (100% over 28bp)
GGCAGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA190990.20350327385718672No Hit
GGATGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA177880.18953433349241516No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATTATGGAATTCTCGGG164780.17557604830717433No Hit
TCGTGGTTGTAGTCCGTGCGAGAATACCATGGAATTCTCGGGTGCCAAGG153200.16323735041060267Illumina Small RNA Adapter 2 (100% over 21bp)
GGGTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA149550.15934820988189052No Hit
TGAGGGGCAGAGAGCGAGACTTTTGGAATTCTCGGGTGCCAAGGAACTCC148560.15829334710834944RNA PCR Primer, Index 1 (100% over 27bp)
TATTGCACTTGTCCCGGCCTGTATTGGAATTCTCGGGTGCCAAGGAACTC148290.15800565726102003RNA PCR Primer, Index 1 (100% over 26bp)
TCCTGTACTGAGCTGCCCCGATGGAATTCTCGGGTGCCAAGGAACTCCAG145640.15518203468537972RNA PCR Primer, Index 1 (100% over 29bp)
TCCTGTACTGAGCTGCCCCGAGTTGGAATTCTCGGGTGCCAAGGAACTCC135850.14475061392480662RNA PCR Primer, Index 1 (100% over 27bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCAA134830.14366378561267334No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTATGGAATTCTCGGGTGC129190.13765426435734832No Hit
AGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA125430.13364791685379826No Hit
GCTCTACACTCTACTCGTCAGCTGTTGGAATTCTCGGGTGCCAAGGAACT118140.1258802909759047RNA PCR Primer, Index 1 (100% over 25bp)
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCACGGGTGCCA109450.11662093996371058No Hit
ATTCCCGTGTGAAAGTAGGTAATCGTCAGGCTTGGAATTCTCGGGTGCCA108370.11547018057439302No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTAGGGTGCCA105620.11254000620344552No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGACA104630.11148514342990443No Hit
AGATAGTACTGATCTGCTGCGACGAGTGACTGTCTGGAATTCTCGGGTGC100040.10659441602530477No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTCTAGGGTGCCAA99070.10556086361082512No Hit
GGCTGGTCCGATGGTAGTGGGTTATCAGAACTGGAATTATCGGGTGCCAA97730.10413306955370887No Hit
CGCTGGTCCGATGGTAGTGGGTTATCAGAACTTGGAATTCTCGGGTGCCA97380.10376013827013374No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAGTCAAATCTCGTATGC96170.10247086154691683RNA PCR Primer, Index 13 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGGT6309950.069.944941
GCTGGTC6385250.069.8074952
CTGGTCC6401150.069.793763
TGGTCCG6541650.069.790244
GGTCCGA6604250.069.711695
GTCCGAT6573900.069.660296
TGTCCCG49850.069.6495210
TCCGATG6577900.069.6073157
GTGGTCC43850.069.604083
TGAGGTA117550.069.526771
AGTGGGT6617250.069.52650516
CGATGGT6605200.069.526499
TAGTGGG6618700.069.515515
TGGGTTA6677650.069.50960518
GGGTTAT6678550.069.5070619
GGTAGTG6616800.069.5021313
GATGGTA6616950.069.49860410
ATGGTAG6617150.069.493711
GAACTTG5671450.069.49280528
GTGGGTT6680400.069.49184417
Produced by FastQC (version 0.11.5)